Objective:Through the detection of mycoplasma pneumoniae (MP) fast culture medium,make sure the infection of MP in children and help the diagnosis in clinic. Method:Use the MP fast culture medium to detect the respiratory secretion of the child patients who got the respiratory tract infection during the Janurary 2009 to Janurary 2010 in Luzhou People’Hospital.Result: The MP fast culture medium shows 58 cases in 432 children in respiratory infection is positive,the positive rate is 13.43%.Result:The mycoplasma often can be found in children,it is very important to use the MP fast culture medium to detect it and to avoid the severe condition and the organs damage.